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KMID : 1145720140020020057
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Korean Herbal Medicine Informatics
2014 Volume.2 No. 2 p.57 ~ p.66
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Exploration of Proteins and Analysis of Valuable Compounds from Korean Herbal Medicine as known Siberian Ginseng (Eleutherococcus sessiliflorus, E. nodiflorus, and E. divaricatus var. chiisanensis)
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Shin Hwang
Ju Jeong-Yun
Kim Hee-Soo
Yim Jae-Yeop
Lee Jeong-Yeon
Choi Yoon-Hee
Kang Young-Min
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Abstract
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We analyzed Korean Herbal Medicine as known Siberian Ginseng (Eleutherococcus sp.) for valuable proteins by using TLC, HPLC, and SDS-PAGE. This experiment's target is analogizing useful compounds and protein elements which are included in the three Eleutherococus sp. And the main ingredients were the standard compounds : Eleutheroside, Sesamin, and Chiisanoside. First, we made plants' specimens to keep out and performed TLC and HPLC after sampling of specimens. Each extraction liquids of leaf, stem, and root from Eleutherococus sp. were differentially contributed. Chiisanoside was included in leaf of all three Eleutherococus sp. and Sesamin was involved in stem/root of all three Eleutherococus sp. However, Eleutheroside (E) was not detected in leaf of all three species, but detected on the root/stem of E. sessiliflorus and E. divaricatus var. chiisanensis.
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KEYWORD
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Eleutherococcus sp., HPLC (High Performance Liquid Chromatography), Proteomics, SDS-PAGE (Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis), TLC (Thin-layer Chromatography)
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